Part:BBa_K145001

T7 RNA polymerase

This RNA polymerase will start transcription from a T7 promoter (such as BBa_I712074).

BOKU-Vienna 2019 - Improvement

Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under BBa_K3015006

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_K145001 parameters

Burden Imposed by this Part:

Burden Value: 1.5 ± 3.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.